|
OJHAS Vol. 11, Issue 1:
(Jan-Mar 2012) |
|
|
| Seroprevalence of HIV, HBV, HCV
and Syphilis in
Blood Donors in Saurashtra Region of Gujarat: Declining Trends Over
a Period of 3˝ Years |
|
Dhruva Gauravi A, Professor & Head,
Agravat Amit H, Associate Professor,
Pujara Krupal M, 2nd year Resident,
Department of Pathology, PDU Medical College, Rajkot, Gujarat. |
|
|
|
|
|
|
|
|
|
Address for Correspondence |
“Matru Ashish”,
3, Navjyot Park,
Kalavad Road, 150ft Ring Road,
Rajkot-360 005, Gujarat.
E-mail:
path.pdumc@gmail.com |
|
|
|
|
|
Dhruva GA, Agravat AH, Pujara KM. Seroprevalence of HIV, HBV, HCV
and Syphilis in
Blood Donors in Saurashtra Region of Gujarat: Declining Trends Over
a Period of 3˝ Years. Online J Health Allied Scs.
2012;11(1):5 |
|
|
|
|
Submitted: Jan 16,
2012;
Accepted: Mar 10, 2012; Published: Apr 15, 2012 |
|
|
|
|
|
|
|
|
|
| Abstract: |
|
Background: Transfusion of blood
and blood products is a life saving intervention and benefits innumerous
patients worldwide. At the same time it could be an ominous mode of
infection transmission to recipients. In 15 percent of total patients
infected with HIV, blood transfusion has been the responsible mechanism
of transmission. Methods: In this study, we aimed
to access the prevalence and trend of HIV, HBV, HCV and Syphilis over
the last 3˝ years (January 2008 to June 2011) among the blood donors
who came to donate blood at Blood Bank, P.D.U. Medical College &
Hospital, Rajkot as well as in various blood donation camps organized
by the same blood bank. Results: From the total of 30,178
blood donors, 711 (2.35%) had serological evidence of infection with
at least one pathogen, either of HIV, HBV, HCV or Syphilis. These included
131 (0.43%) with HIV, 293 (0.97%) with HBV, 124 (0.41%) with HCV and
94 (0.31%) with Syphilis. Moreover, significantly declining trends of
HIV, HBV and Syphilis was observed over the study period.
Conclusion: A substantial percentage
of blood donors harbor HIV, HBV, HCV and Syphilis infections. Strict
selection of blood donors and comprehensive screening of donors’ blood
using standard methods are highly recommended to ensure the safety of
blood for recipient.
Key Words:
Blood donors; HIV;
HBV; HCV; Syphilis; Transfusion transmissible infection (TTI).
|
|
Everywhere in this world, transfusion of human blood is an essential therapeutic
procedure, as there is no genuine substitution. Even though it can save human lives, in some instances it can transmit infectious
diseases, which are fatal. Especially with the rapid increase in number of people with transfusion transmissible infections (TTI’s)
including HIV, Hepatitis B and C, HTLV I & II, Syphilis, CMV, EBV, Brucellosis, Toxoplasmosis, etc. the field of transfusion medicine
has encountered a huge problem in providing safe blood and blood products; therefore there is a need to improve testing for transfusion
transmissible diseases and the selection of blood donors. The aim of the present study was to know the seroprevalence
of transfusion transmissible infections in donors in this area as the incidence
of transfusion transmitted HIV & Hepatitis is increasing in India. [1]
The present study was conducted at Blood Bank, P.D.U.
Medical College & Hospital, Rajkot. The Blood Bank is located in the Civil Hospital of Rajkot City which is considered
to be the capital of Saurashtra region of Gujarat with an annual average donation of 8600 blood bags. Tests are routinely
done on every blood unit to exclude HIV, HBV, HCV, Syphilis and Malaria. Data was collected over a period of 3˝ years from
January 2008 to June 2011. In a 3˝ years period 30,178 donors were tested. Donors were selected by the standard criteria for
donor fitness. The screening for HIV was done by ELISA (Standard Diagnostics (SD), India), HBsAg was detected by ELISA
(Hepalisa, J.Mitra and Co. India), Anti-HCV test was done by ELISA (Standard Diagnostics (SD), India and Qualisa, Tulip
Diagnostics, India). Test for Syphilis was done by RPR method (Span Diagnostics, India). All the reactive samples were
repeated in duplicate as recommended by NACO (National AIDS Control Organization). Repeat reactive were labeled as ELISA
positive for respective infection and were discarded. Further, within the seroreactive group, cases with a combination of
≥2 TTI’s were labeled as co-infection. The number, type and distribution of co-infections were noted and the findings were
analyzed.
Yearly distribution of detected seropositivity is given in
Table 1. Table 2 gives the details of sex and type of seropositive donors.
|
Table 1: Yearly distribution of seropositive blood donors. |
Year |
Total Units |
HIV positive N (%) |
HBsAg positive
N (%)
|
Anti-HCV positive
N (%)
|
RPR positive
N (%)
|
2008 |
7882 |
60 (0.76%) |
107 (1.36%) |
32 (0.40%) |
27 (0.34%) |
2009 |
8141 |
32 (0.39%) |
99 (1.22%) |
24 (0.29%) |
23 (0.28%) |
2010 |
9441 |
29 (0.31%) |
108 (1.14%) |
39 (0.41%) |
35 (0.37%) |
Jan-Jun 2011 |
4714 |
10 (0.21%) |
48 (1.01%) |
29 (0.62%) |
09 (0.19%) |
The co-infection rate was highest in 2008 with 8 cases, out of which 3 were HIV+HBV;
with other 3 of HIV+HCV followed by 1 case each of HIV+Syphilis and HCV+Syphilis. The rate dropped down to 2 cases in year 2009 and
2010 and zero case in 2011 (Jan to June).
|
Table 2: Sex and type of seropositive
blood donors |
|
Year |
Distribution |
HIV N (%) |
HBV N (%) |
HCV N (%) |
Syphilis N (%) |
|
2008 |
Male |
58 |
103 |
31 |
27 |
|
Female |
02 |
04 |
01 |
00 |
|
Voluntary |
19 (31.67 %) |
31 (28.98%) |
12 (37.5%) |
14 (51.85%) |
|
Replacement |
41 (68.33%) |
76 (71.02%) |
20 (62.5%) |
13 (48.15%) |
|
2009 |
Male |
31 |
95 |
23 |
23 |
|
Female |
01 |
04 |
01 |
00 |
|
Voluntary |
11 (34.38%) |
43 (43.43%) |
10 (41.67%) |
09 (39.13%) |
|
Replacement |
21 (65.62%) |
56 (56.57%) |
14 (58.33%) |
14 (60.87%) |
|
2010 |
Male |
29 |
104 |
38 |
34 |
|
Female |
00 |
04 |
01 |
01 |
|
Voluntary |
08 (27.59%) |
43 (39.81%) |
15 (38.46%) |
15 (42.86%) |
|
Replacement |
21 (72.41%) |
65 (60.19%) |
24 (61.54%) |
20 (57.14%) |
|
January-June 2011 |
Male |
10 |
43 |
27 |
09 |
|
Female |
00 |
05 |
02 |
00 |
|
Voluntary |
01 (10%) |
06 (12.5%) |
02 (6.90%) |
03 (33.33%) |
|
Replacement |
09 (90%) |
42 (87.5%) |
27 (93.10%) |
06 (66.67%) |
Transfusion Transmissible Infections (TTI’s) continue to be a threat to safe transfusion practice.
With every unit of blood, there is 1% chance of transfusion associated problem including TTI’s.[2]
Acquisition of HIV disease through blood transfusion is a relatively efficient
mode of transmission, with rates approaching 100%.[3] In 15 percent of total
patients infected with HIV, blood transfusion has been the responsible mechanism
of transmission.[4] Stringent
screening of donors for transfusion transmissible infections is crucial to ensure safe supply of blood and blood products. Our
study was dominated by replacement donors in whom all infections were found to be more prevalent. The size of voluntary donors was
smaller and infections slightly less prevalent. Voluntary donors mainly consisted of students, religious groups and voluntary
organizations. Females made a smaller section of the study as they were found to be anemic and did not fulfill the required
fitness criteria. The prevalence of all infections was found to be low among females.
In our study, HIV, HBV and Syphilis prevalence among donors shows a
downward trend over the period of 3˝ years. With the use of newer generation kits and increase in knowledge about these diseases in
public (opt for early detection), the hospital data among the patients shows an increasing trend in these diseases over the period.
The declining trends in seroprevalence of various TTI’s in the blood donors is a good signal for the society as the risk of acquiring
infections due to transfusion is decreased. The declining trends may be due to the public awareness created by mass media to encourage
voluntary donors to self-deferral if they belong to any of the high risk groups. Also the NACO (National AIDS Control Organization)
strives very hard in providing latest generation kits for the detection of TTI’s so that the transfusion practice remains nearly 100%
safe. Low seropositivity for diseases in our study could be attributed to proper counseling of blood donors and donor selection criteria.
As well as GSACS (Gujarat State AIDS Control Society) is making everyone well aware by promoting blood donation activities as well as Blood Banks.
To conclude, voluntary blood donation should be encouraged for prevention of Transfusion Transmissible
Infections (TTI’s). The time and cost involved in screening donated blood can be reduced by an effective donor education
and selection program that promotes self exclusion by donors at risk of TTI’s. Media in general has reached to public
in a way that the donor is well aware of pre and post events as well as care after blood donation at individual level. The
seropositivity could be further decreased by introduction of nucleic acid amplification testing (NAT) in minipools for HCV and
HIV and introduction of anti HBcAg (IgM) for HBV infection.[5,6] But this may not be possible in near future in developing
countries like India due to financial constraints.[7] The present study concluded that motivating voluntary blood donors is the
most effective way of ensuring adequate supplies of safe blood on a continuing basis.
- Dolly R, Annie S, Thaiyanayaki P, Babu George P, Hohn Jacob T. Increasing prevalence of HIV antibody among
blood donors monitored over 9 years in blood bank. Indian J Med Res 1998;108:42-44.
- Garg S, Mathur DR, Garg DK. Comparison of seropositivity of HIV, HBV,
HCV and Syphilis in replacement and voluntary blood donors in western India.
Indian J Pathol Microbiol 2001;44:409-412.
- Ward JW, Deppe DA, Samson S, Perkins H, Holland P, Fernando L, et al. Risk of
HIV infection from blood donors who later developed the acquired immunodeficiency syndrome.
Ann Intern Med 1987;106:61-62.
- Lal S. Monthly update on HIV infection in India.
Center AIDS Res Contr. 1993;6:133-134.
- Kaur P, Basu S. Transfusion transmitted infections: Existing and emerging pathogens.
Journal of Post Graduate Medicine. 2005;51:(2):146-151.
- Glynn SA, Kleinman SH, Wright DJ, Busch MP. For the NHLBI Retrovirus Epidemiology
Donor study: International application of the incidence rate/window period model.
Transfusion 2002;42:966-972.
- Jackson BR, Busch MP, Stramer SL, AuBuchon JP. The cost-effectiveness of NAT for HIV,
HCV and HBV in whole-blood donations. Transfusion 2003;43:721-729.
|
